With the growth of precision medicine and the development of personalized medicine, the number of available assays has been increasing along with the cost of healthcare. Multiplexing provides an attractive solution for cost-reduction, as unlike traditional methods, a multiplex assay can provide results for multiple targets in a single reaction, saving time, labor, reagents, samples, and thereby reducing healthcare costs.
PlexBio’s Precision Image Code ( πCode ) MicroDiscs are manufactured by semi-conductor processes and able to generate over 16,000 distinct circular image patterns for multiplexing applications. To be used in biological applications, each circular microdisc is encapsulated in a highly stable polymer with an added paramagnetic property, which allows it to be suitable for bio-conjugation, washing, and automation applications.
Each πCode MicroDisc has a distinct circular image pattern, which corresponds to the specific capture agents conjugated to the disc surface, thus allowing the capture and detection of specific analytes from a sample. Virtually any probe used in clinical diagnostics can be conjugated to πCode MicroDiscs, including DNA, RNA. antigens, antibodies, proteins, or chemical compounds.
In order to perform a biological test, πCode MicroDiscs with desired detection probes were pooled together in a single well and reacted with patient sample, followed by hybridization and fluorescent labeling. The assay detection was done by optical imaging florescence analyzer PlexBio 100/200, which uses the CCD camera to read the distinct image patterns under bright field and quantifies the analyte by reading the fluorescence signal intensity under dark field, reporting the reactions occurring at each πCode MicroDisc in a user-friendly interface.